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A 0.88 kb fragment spanning the start codon and the remainder of exon 3 was replaced by a 1.7 kb PGKneo cassette. A 4.5 kb EcoRI-ApaI genomic fragment was used as the left arm for homologous recombination and was cloned into the pPGKneo vector upstream of the PGKneo cassette. Northern blot analysis and QPCR confirmed the absence of transcription. (J:107617)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
