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EN
Exon 2 was replaced with a mutated exon 2 encoding 7 amino acid substitutions (H46E, S47A, S51A, E52R, K53D, D79K, R80E) in three regions of the amino-terminal CUB domain, and a loxP site flanked neomycin resistance gene cassette was inserted into intron 1. The neo cassette was removed through subsequent cre-mediated recombination. Mutation of these sites was shown to abolish Sema3 family binding but not VEGF binding. Immunohistochemistry showed that homozygotes express normal levels of protein. (J:93799)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
