Tg(Krt1-15-EGFP)2Cot

The transgene comprises the entire 5.0-kb DNA segment that separates the Krt1-19 and Krt1-15 genes in the mouse genome and that contains the Krt1-15 promoter region joined to the cDNA encoding enhanced green fluorescent protein (EGFP). The Krt1-15 promoter directs expression to the bulge region of the hair follicle; fluorescence activated cell sorting (FACS ) analysis demonstrated that highest EGFP expression occurs in bulge cells showing high surface expression of the epithelial stem cell marker integrin beta 1 (fibronectin receptor beta). (J:94076, J:94082)