Exons 2-4 were replaced with a neomycin resistance gene by homologous recombination. RT-PCR, Northern blot and Western blot analyses confirmed absence of transcript and protein in mutant livers and colons. (J:93428)
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Exons 2-4 were replaced with a neomycin resistance gene by homologous recombination. RT-PCR, Northern blot and Western blot analyses confirmed absence of transcript and protein in mutant livers and colons. (J:93428)