Gfi1^tm2Tmo

A targeting vector was designed to replace exons 3-5 with an in-frame GFP and insert a floxed neo. Crossing with mice expressing Cre recombinase in the germ line removed the neomycin resistance gene. Fluorescence was detectible in virtually all thymocytes of mutant mice. Western blot showed that GFP expression in mutants was similar to that of gene expressed in wild-type mice. (J:93337)