Btrc^tm1Paga

The targeting event deleted a large segment of exon 5 that encodes amino acids 154-212 of the protein, including all but four amino acids comprising the F box and 22 downstream amino acids. The neomycin resistance gene is inserted in reverse orientation, and stop codons occur in all three reading frames within exon 5 following codon 153. The splice acceptor site of exon 5 is intact, and exons 6 and 7 are both out of frame with exon 4. No expression was detected in homozygous mutant mouse embryonic fibroblasts or other tissues examined by Northern blot analysis or by immunoprecipitation followed by immunoblot analysis. (J:93010)