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The targeting construct includes the signal sequence, first intron and propeptide of the mouse gene, the equivalent human cDNA and a neomycin cassette. Two site specific point mutations, T148A and R333Q were created in the human cDNA. The human cDNA is joined to the mouse gene at codons 6 and 7, such that the mouse expresses a missense mutant human protein, but does not express the mouse protein. ELISA analysis using specific human anti-F9 polyclonal antibodies detects human F9 in homozygous mice. (J:92964)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
