A targeting vector was designed to replace part of exon 2 and all of exons 3-8, encoding the homeodomain and including the polyadenylation signal, with a Pgk1-HPRT cassette. Southern blot and multiplex PCR confirmed recombination in mice. (J:92421)
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A targeting vector was designed to replace part of exon 2 and all of exons 3-8, encoding the homeodomain and including the polyadenylation signal, with a Pgk1-HPRT cassette. Southern blot and multiplex PCR confirmed recombination in mice. (J:92421)