An IRES-cre-FRT-pgk-hygro-FRT cassette was inserted into exon 2. Crossing with Flp expressing mice excised the pgk-hygro sequences. PCR confirmed recombination in mice. Cre expression mirrored expression of the endogenous gene. (J:92294)
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An IRES-cre-FRT-pgk-hygro-FRT cassette was inserted into exon 2. Crossing with Flp expressing mice excised the pgk-hygro sequences. PCR confirmed recombination in mice. Cre expression mirrored expression of the endogenous gene. (J:92294)