The pGt1lxf gene trap vector, which integrated randomly into the first intron - which interrupts the coding sequence within the tenth codon - contains a splice acceptor sequence upstream of a Beta-geo (beta-galactosidase/neomycin phosphotransferase II fusion) reporter gene. Transcription from the endogenous promoter of the trapped gene and splicing of the message yields a fusion mRNA joining exogenous upstream exons to the Beta-geo coding sequence. (J:94077)
Basic Information
Insertion of gene trap vector
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count