The RVIII region was deleted and replaced with an FRT sequence and a loxP flanked PGK-neo in reverse transcriptional orientation about 700 bp downstream of the deleted sequence. Southern blotting verified correct recombination. (J:41828)
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The RVIII region was deleted and replaced with an FRT sequence and a loxP flanked PGK-neo in reverse transcriptional orientation about 700 bp downstream of the deleted sequence. Southern blotting verified correct recombination. (J:41828)