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A floxed neo was inserted in intron 5 and a loxP site in intron 7. Transient Cre expression deleted the larger floxed region, including exons 6 and 7. These exons encode amino acids 254-380, which comprise the N-terminal portion of the rel DNA-binding domain (DBD). Germ line transmission was verified by PCR and Southern blot. RNA and protein expression in mutant MEFs showed that the allele encodes a truncated protein containing an internal deletion as expected. Immunoreactivity was eliminated as demonstrated by using antisera against the DBD. (J:91482)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
