An IRES-lacZ-neo-pA cassette was inserted to disrupt exon 2, the first coding exon. Southern blot and PCR confirmed recombination. Western blot indicates comparable levels of proteins in wild-type and heterozygous ES cells. (J:91600)
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An IRES-lacZ-neo-pA cassette was inserted to disrupt exon 2, the first coding exon. Southern blot and PCR confirmed recombination. Western blot indicates comparable levels of proteins in wild-type and heterozygous ES cells. (J:91600)