Rasd2^tm1Rdl

A targeting vector was used to insert a stop codon in each frame immediately downstream of the endogenous translational start codon. An IRES-EGFP was placed downstream of the endogenous regulatory sequences and a floxed PGKneo were also inserted. Southern blot confirmed recombination. In situ hybridization showed that mRNA was barely detectable in the striatal region of mutant brains. In situ hybridization using an antisense riboprobe of EGFP shows that EGFP mRNA was detected in mutants. Western blotting of striatum protein extracts showed putatively nonspecific bands that were present in both wild-type and knockouts. Therefore, the mutated allele produces no detectable Rasd2 protein. (J:91597)