The entire open reading frame was replaced with a floxed neo cassette via homologous recombination. RT-PCR and western blot analysis confirmed the absence of RNA and protein expression in homozygous mutants. (J:91621, J:91622)
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The entire open reading frame was replaced with a floxed neo cassette via homologous recombination. RT-PCR and western blot analysis confirmed the absence of RNA and protein expression in homozygous mutants. (J:91621, J:91622)