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The DQ52 element of Igh-D was disrupted by the insertion of a floxed neo cassette and the Igh-J elements were replaced with sequence encoding the variable region of DNA binding antibody D42. In constrast to Ightm3Cgn, this allele contains Igh-V11 mutations resulting in a Ser to Asn substitution at position 35 in CDR1 and an Asn to Tyr substitution at position 53 in CDR2. The Igh intron enhancer was left intact. Flow cytometry verified cell surface expression of the mutant protein product. The floxed neo cassette was removed via cre-mediated recombination. (J:76117)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
