A single loxP site was introduced into intron 1 and a floxed neo was inserted upstream of exon 1. Transient cre excised the floxed neo, leaving floxed exon 1. Southern blot confirmed recombination and Northern blot demonstrated presence of RNA. (J:90737)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count