A lacZ with a nuclear localization signal and a pMC1 neo polyA fragment were inserted in-frame into the exon containing the first methionine via homologous recombination. Northern and western blot analysis confirmed the absence of RNA and protein expression in homozygous mutants. LacZ expression was detected in the seminiferous tubules. (J:90439)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129S4/SvJae
Targeted
Insertion
--
1
--
6

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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