An in-frame lacZ gene and neo cassette were inserted after the first 4 codons deleting 1907 bases via homologous recombination. Northern and western blot analysis confirmed the absence of mRNA and protein expression in homozygous mice. (J:89669)
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An in-frame lacZ gene and neo cassette were inserted after the first 4 codons deleting 1907 bases via homologous recombination. Northern and western blot analysis confirmed the absence of mRNA and protein expression in homozygous mice. (J:89669)