Tcf7l1^tm2Efu

A loxP flanked 64 bp segment of exon 2 and a neo cassette were inserted via homologous recombination then removed via cre mediated recombination prior to generation of chimeric mice. The resulting allele lacked exon 2 and contained a frameshift and early termination codon. Immunoblot analysis confirmed the absence of protein expression in homozygous embryos (J:90402)