A single loxP site was inserted into intron 2 and a loxP-flanked neomycin selection cassette was inserted into intron 4 through homologous recombination. Western blot analysis on extracts derived from splenocytes of homozygous mice confirmed that no detectable protein was expressed from this allele. (J:89322)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count