Ms4a1^tm1Tft

Part of exon 6 and all of exons 7-8 were replaced with a neomycin resistance gene via homologous recombination. These exons encode part of the second extracellular loop, the fourth transmembrane domain and the large C-terminal cytoplasmic domain of the endogenous protein. The resulting protein was truncated at amino acid 157 and was fused with an 88-amino-acid peptide coded for by the neo gene promoter sequence. Absence of cell-surface protein expression was verified by flow cytometry. (J:88933)