A gene trap vector inserted into the endogenous gene such that the beta-galactosidase and neomycin genes were fused to the expressed mRNA at position 1149. Beta-galactosidase expression was noted in a similar pattern to the wild-type mRNA. RT-PCR analysis indicated that a low level of wild-type transcript was still expressed from this allele, suggesting that this mutation is a hypomorph. (J:69466)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Insertion of gene trap vector
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count