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A targeting construct was designed to insert sequence encoding IRES, floxed neo, and polyomavirus middle T antigen (PyVT) in the 3' untranslated region of beta actin, 55 bp downstream of the stop codon. The inserted cassette leaves the endogenous coding region and the 54 bp 'zipcode' that directs cellular localization of beta actin intact. PyVT expression is dependent on the removal of the floxed neo cassette (which occurred at low frequency without cre expression). (J:87544)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
