Exons 1 and 2 were replaced with a floxed Pgk-hygromycin cassette inserted in reverse orientation. Absence of transcript and protein product was established by Northern blot analysis and immunofluorescence experiments respectively. (J:87577)
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Exons 1 and 2 were replaced with a floxed Pgk-hygromycin cassette inserted in reverse orientation. Absence of transcript and protein product was established by Northern blot analysis and immunofluorescence experiments respectively. (J:87577)