Omp^tm2(spH)Mom

The coding exon was replaced by a construct containing the ecliptic synaptopHluorin (spH) coding sequence and a self-excising neomycin resistance cassette. SpH is a fusion protein joining a pH-sensitive derivative of wild-type green fluorescent protein (GFP) to the carboxyl terminus of a mouse synaptic vesicle-associated membrane protein (VAMP2), which targets spH to the lumenal face of pre-synaptic vesicles. The loxP-flanked, male-germline self-excising neomycin resistance cassette (which was deleted in the chimeric founder's germline) contains the Cre recombinase coding sequence under control of the mouse testis-specific angiotensin-converting enzyme (tACE) promoter and tk-neo. The endogenous gene regulatory sequences direct spH/Vamp expression specifically to sensory neurons in glomeruli of the olfactory bulb. (J:36595, J:79620, J:88765)