Hmmr^tm1Baa

A genomic fragment encompassing exons 8 through 16 was replaced with a Hprt minigene. Full length transcript was undetected by RT-PCR analysis of homozygous mutant spleen tissue. Analysis of homozygous mutant MEFs identified aberrant transcript in which exon 7 had spliced to exon 17. Sequence analysis indicated a frameshift mutation at the aberrant splice junction, affecting downstream sequence encoding the HA-binding region. Western blot analyses of homozygous spleen tissue and MEFs did not detect protein corresponding with the mutant transcript. (J:86065)