The promotor region and the 5' end of the coding region were replaced by a neomycin resistance gene in inverse orientation. Absence of mRNA transcripts was demonstrated by RT-PCR and absence of protein product by Western blot. (J:85937)
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The promotor region and the 5' end of the coding region were replaced by a neomycin resistance gene in inverse orientation. Absence of mRNA transcripts was demonstrated by RT-PCR and absence of protein product by Western blot. (J:85937)