Sirt1^tm2.1Fwa

A single loxP site remained in place of exons 5, 6, and 7 after the floxed neo cassette inserted by homologous recombination was excised via in vitro cre-mediated recombination. Protein was undetected in homozygous mutant MEFs by Western blot analysis using an antibody specific for the N-terminal. (J:85542)