Tg(Itga2b-cre)NE1/21Emam

The transgenic construct was comprised of an integrin alpha 2b genomic fragment, including 2.5 kb of promoter through 2.8 kb of intron 1, adjoined to sequence encoding cre recombinase with a nuclear localization signal. The cassette containng NLS-cre also contained a splice acceptor site, IRES, and a polyadenylation signal. The integrin alpha 2b promoter drives expression of cre recombinase at the site of emergence of hematopoietic progenitors in the yolk sac, aorta-gonad-mesonephros region, and fetal liver. Founder mice contained between 2 and 5 copies of the transgene. (J:84564)