Thra^tm2Ven

An SV40 polyadenylation signal followed by a thymidine kinase/neomycin selection cassette was inserted into exon 10 in order to disrupt the alpha2 isoform, while maintaining the integrity of the alpha1 isoform. The last exon of the RevErbAalpha gene that overlaps with Thra exon 10 (TRalpha2-specific) on the opposite strand was unaffected. Northern blot analysis demonstrated that no detectable transcript for the alpha2 isoform was present in adult brain. The mutation leads to concomitant overexpression of alpha1 as a result of the structure of the 3' exons of the gene. (J:72959)