Tg(Prnp)F35Cwe

A transgenic construct was engineered to contain murine Prnp lacking sequence encoding amino acids 32 through 134. Sequence, including that encoding the amino-terminal signal sequence, necassary for proper transport to the endoplasmic reticulum and proper processing was left intact. Transgenic expression was verified by Western blot analysis. Only after treatment with PNGase did the transgenic protein migrate a distance corresponding to the deletion, indicating in vivo glycosylation. A carboxy terminal cleavage product was also detected. (J:47034)