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An EGFP reporter gene followed by a single loxP site was inserted into intron 1 in reverse transcriptional orientation to the gene. A neomycin selection gene followed by a single loxP site and a thymidine kinase promoter were inserted into intron 7. Cre mediated recombination in the germ line deleted exons 2-7 and brought the thymidine kinase promoter into proximity of the EGFP gene to activate its transcription. (J:69949)