Prkg2^tm1Pfe

A 308 bp fragment of the second coding exon was replaced with a neomycin selection cassette inserted by homologous recombination. The disrupted exon encoded a portion of the cGMP binding pocket. Northern blot, RT-PCR, and Western blot analyses indicated an absence of normal transcript and protein in homozygous mutant mice. Functional ablation was confirmed by a lack of cGMP-stimulated phosphotransferase activity in jejunal homogenates. (J:37306)