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The gene was disrupted by replacement of a 4.5 kb region of the gene containing a 5' non-coding exon and the first 4 exons encoding amino acids 1-333. Absence of gene expression was confirmed by Western blot analysis of lung and spleen mRNA from homozygous mutant animals. Multiple antibodies directed against N-terminal, central, and C-terminal residues did not detect any protein product. (J:81007)