Sox2^lcc

A mutation recovered in a radiation mutagenesis experiment. Molecular analysis revealed an inversion with breakpoints at B and E1. Genetic noncomplementation tests with a targeted mutation indicated that this mutation is an allele of Sox2. Genomic PCR analysis showed that the coding region and nearby flanking DNA was intact in the mutants. Expression of the gene was intact in most of the developing neural tube but was not detected in the otocyst or adjacent region of the hindbrain, in contrast to controls. This tissue specific loss is thought to be due to loss or translocation of tissue-specific regulatory elements. (J:98458)