Igf2r^tm1.1Rlj

The floxed fragment containing exon 10 was excised from Igf2rtm1Rlj via in vivo cre mediated recombination. Sequence analysis indicated that the deletion of exon 10 will cause a shift in the open reading frame resulting in a stop codon upstream of the mannose 6-phosphate binding site, the Igf2 protein product binidng site, and the transmembrane domain. While, protein was undetected in mutant embryos by Western blot analysis, immunohistochemical analysis showed protein expression in developing bronchiolar structures. This staining putatively represents expression from an unsilenced paternal allele. (J:80932)