A construct containing a floxed neo cassette, Pax5 gene, and GFP gene was inserted into the Znfn1a1 locus upstream of the translation initiation codon in exon 2. Western blot analysis using antibodies against C-terminal Znfn1a1 sequences did not detect protein product in thymocytes from homozygous mutant animals. Trace amounts of Pax5 protein was detected in thymocytes. (J:80815)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count