Tg(BLG-STAT5A)15Itb

The transgene consists of a beta-lactoglobulin promoter, active in mammary glands, and a fusion gene comprised of amino acids 1-750 of the sheep STAT5A gene, the terminal transactivation domain (amino acids 677-847) of human STAT6, and the kinase domain (amino acids 757-1129) of mouse Jak2. The resulting protein is a constitutively active STAT5a molecule with self-activating properties. Transgene expression was confirmed by Western blot and RT-PCR analysis of mammary glands from transgenic animals at day 6 of lactation. (J:80588)