Tg(PDGFB-APOE)1Viz

A 4.9 kb genomic fragment containing exons 1 through 4 of the human APOE gene was adjoined to a 1.4 kb human PDGFB promoter fragment. Epsilon 2 (APOE2) was identified at both the mRNA and protein levels by RNase protection and Western blot analysis of transgenic brain tissue. Immunohistochemical analysis further localized the transgenic expression to hippocampal neurons. (J:78003)