Tg(hAGT)2041Sig

The 14 kb transgenic construct contained the entire human angiotensinogen gene (approximately 11.5 kb) and 5' (1.2 kb) and 3' (1.4 kb) flanking sequence. Sequence analysis showed that the construct contained the M235T variant but not the T174M variant. The human AGT promoter drove high levels of expression in the liver and kidney. Expression was also detected in the heart, adrenal gland, and adipose tissue, and at very low levels in the brain and submandibular gland. Sexually dimorphic expression, in which expression was higher in males, was observed in the kidney and adrenal gland. Lines 204.1 (2041) and 204.3 (2043) were created and transgenic mice on a C57BL/6 background show angiotensinogen plasma concentrations 150X that normally found in human plasma. (J:79944)