The entire coding region was replaced with a PGK-neo cassette via homologous recombination. The absence of endogenous protein was demonstrated by Western blot and immunohistochemical analyses of homozygous mutant mice. (J:75781)
查看原文 参与反馈
The entire coding region was replaced with a PGK-neo cassette via homologous recombination. The absence of endogenous protein was demonstrated by Western blot and immunohistochemical analyses of homozygous mutant mice. (J:75781)