Camk2a^tm5Sva

Sequence encoding threonine 305 was targeted by homologous recombination of a construct containing an engineered arginine substitution mutation. The replacement of the this threonine residue with the negatively charged argnine was designed to interfere with calcium/calmodulin binding. Western blot analysis confirmed the expression of a normal sized protein. A single loxP site was left within the locus. (J:79714)