Tagln^{tm1(cre/ERT2)Feil}

An allele encoding a tissue specific and ligand dependent recombinase was generated by inserting cre recombinase/modified estrogen receptor (ESR1) fusion sequence into the endogenous allele. The ESR1 sequence contained engineered missense mutations at positions 400, 543, 540 which substituted the ability to bind the endogenous ligand with the ability to bind tamoxifen (J:79835). Histochemical analysis of mutant mice treated with tamoxifen showed expression of cre in smooth muscle tissue. (J:69325)