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The gene was disrupted using a two-step targeting strategy. First, exons 2-10 were replaced with a neo-TK cassette via homologous recombination. Next, correctly targeted ES cells were subjected to another round of gene targeting to replace the neo-TK cassette with a lacZ cassette. Successful gene replacement was selected for via gancyclovir resistance. Homozygous mutant animals were successfully established. (J:79629)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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| Phenotypes |
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References Literature
Title
PMID
Journal
Year
IF
