The transgene is composed of a cDNA encoding the long form of murine B4galt1, the mouse Mt1 promoter, and human growth hormone 3' polyadenylation sequences. Intron 4 was included in the B4galt1 sequence to increase transgene expression. Expression of long form B4galt1 was verified by RNase protection assay of testes RNA. Transgene expression was also confirmed by enzyme assays, immunoprecipitation enzyme activity, and Western blot analysis. (J:20319)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(C57BL/6 x DBA/2)F2
--
Insertion
--
1
--
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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