Cbfb^tm1Ppl

MYH11 sequence isolated from a human CBFB-MYH11 cDNA, resulting from a chromosome 16 inversion common in human acute myeloid leukemia subtype M4Eo, was inserted into a StuI site in exon 5 of the endogenous gene by homologous recombination. The targeting vector also contained a neo cassette inserted into intron 5 for selection. Northern and Western blot analyses confirmed the production of fusion transcript and protein in heterozygous mutant embryos. (J:36594)