The gene was disrupted by insertion (knock-in) of a GFP-neo cassette into the coding sequence. RT-PCR analysis of bone marrow RNA confirmed the absence of Rag1 gene expression in homozygous mutant animals. Instead the mutant animals produced GFP transcripts. (J:79416)
Basic Information
(C57BL/6NCrlj x CBA/JNCrlj)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count