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The gene was specifically inactivated in non-bone marrow antigen presenting cells by deleting the pIV promoter and its associated exon 4. A loxP site was introduced upstream of pIV and a floxed neo cassette was introduced downstream of exon 4. Transient expression of cre recombinase in correctly targeted ES cells removed the entire floxed region (800 bp) containing the pIV promoter and exon 4. Ablation of type IV mRNA in Ifng-stimulated MEFs (mouse embyronic fibroblasts) derived from homozygous mutant animals was confirmed by RNase protection assay. Gene expression from the pI and pIII promoters was not affected by the pIV deletion. (J:71102)
Basic Information
Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Phenotypes
Legend：
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes：
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References Literature
Title
PMID
Journal
Year
IF
