Dlg2^tm1Dsb

The exon encoding the second PDZ domain was replaced with a TK-neo cassette via homologous recombination. Northern blot analysis of brain RNA from homozygous mutant animals detected a faint mutant transcript of higher mobility, presumably an alternative splice product that skips the deleted exon. However, Western blot analysis of brain proteins using antibodies directed against a region N-terminal to the deleted exon did not detect protein product. Exon 9 is deleted. In knockout mice, the longer Dlg2gamma and DLG2alpha1 are not expressed in bone-marrow derived plasmacytoid dendritic cells. However, a shorter Dlg2n isoform is expressed in bone-marrow derived plasmacytoid dendritic cells and in brain (J:262606). (J:68924, J:262606)